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Thiazolyl Blue

Thiazolyl Blue
Thiazolyl Blue (MTT) is a colorimetric compound extensively utilized to measure cell proliferation, transitioning from a yellow color to purple formazan in living cells.
Catalog No. T19029Cas No. 298-93-1
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Purity:99.65%
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Thiazolyl Blue

Purity: 99.65%
Catalog No. T19029Alias Thiazolyl Blue Tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromideCas No. 298-93-1

Thiazolyl Blue (MTT) is a colorimetric compound extensively utilized to measure cell proliferation, transitioning from a yellow color to purple formazan in living cells.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
1 g$46In Stock
1 mL x 10 mM (in DMSO)$30In Stock
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Product Introduction

Bioactivity
Description
Thiazolyl Blue (MTT) is a colorimetric compound extensively utilized to measure cell proliferation, transitioning from a yellow color to purple formazan in living cells.
AliasThiazolyl Blue Tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
Chemical Properties
Molecular Weight414.32
FormulaC18H16BrN5S
Cas No.298-93-1
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 50 mg/mL (120.68 mM)
H2O: 0.88 mg/mL (2.12 mM), Sonication and heating are recommended.
Solution Preparation Table
H2O/DMSO
1mg5mg10mg50mg
1 mM2.4136 mL12.0680 mL24.1359 mL120.6797 mL
DMSO
1mg5mg10mg50mg
5 mM0.4827 mL2.4136 mL4.8272 mL24.1359 mL
10 mM0.2414 mL1.2068 mL2.4136 mL12.0680 mL
20 mM0.1207 mL0.6034 mL1.2068 mL6.0340 mL
50 mM0.0483 mL0.2414 mL0.4827 mL2.4136 mL
100 mM0.0241 mL0.1207 mL0.2414 mL1.2068 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
% DMSO
%
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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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